Oocyte Selection for In Vitro Embryo Production in Bovine …
WEBNov 24, 2020 · Research over the past decades indicates that bovine oocytes gain competence at late stages of the follicular phase, when signs of atresia are observed for the first time, such as a slight expansion in the outer cumulus layers and some cytoplasmic granulations [7,32].
Nutritional Strategies to Promote Bovine Oocyte Quality for In …
WEBOct 4, 2023 · In this review, the nutritional strategies of bovine oocyte donors to promote oocyte developmental competence (i.e., ability of the oocyte to reach the blastocyst stage) in vitro will be discussed, with the aim of providing an …
Bovine oocyte maturation: acquisition of developmental …
WEBAn important aspect of oocyte maturation is the segregation of homologous chromosomes and subsequently sister chromatids to form a haploid oocyte. Furthermore, the cytoplasm needs to be prepared for the formation of pronuclei and nuclear reprogramming to form a totipotent zygote.
Nutritional Strategies to Promote Bovine Oocyte Quality for In …
WEBOct 4, 2023 · The ability of bovine oocytes to reach the blastocyst stage (i.e., embryo with around 150 cells in cattle) in vitro can be affected by technical (e.g., culture medium used) and physiological factors in oocyte donors (e.g., age, breed).
Advances in Oocyte Maturation In Vivo and In Vitro in Mammals
WEBMay 21, 2023 · The oocyte, one of the largest cells in the mammalian body, is a female gametocyte (or germ cell) involved in reproduction. The maturation of oocytes has four important stages, namely, the germinal vesicle (GV) stage, germinal vesicle breakdown (GVBD), metaphase I (MI), and metaphase II (MII) [13, 34].
Transvaginal ultrasound‐guided oocyte retrieval in cattle: …
WEBDec 13, 2022 · Transvaginal ultrasound-guided oocyte retrieval (commonly called OPU) and in vitro embryo production (IVP) in cattle has shown significant progress in recent years, in part, as a result of a better understanding of the full …
IVF bovine oocyte classification and selection - Cell Press
WEBFeb 10, 2023 · IVF bovine oocyte classification and selection. The current technology for in vitro fertilization (IVF) has a long history of merely 40 years. The addition of all live births from either fresh or frozen oocytes transferred from a single oocyte collection is known as the IVF productivity rate.
Frontiers | Bovine In Vitro Oocyte Maturation and Embryo …
WEBBovine oocytes were exposed to DES during in vitro maturation (IVM) or embryos were exposed during in vitro embryo culture (IVC). The endpoints evaluated included nuclear maturation, mitochondrial redistribution, cumulus cell expansion, apoptosis, and steroidogenesis.
Simple separation of good quality bovine oocytes using a
WEBSep 24, 2018 · We fabricated a simple microfluidic device for separation of bovine oocytes based on the oocyte quality to improve the conception rate of in vitro fertilization (IVF) by using good quality...
An updated protocol for in vitro bovine embryo production
WEBMar 17, 2023 · We describe a protocol to mature and fertilize bovine oocytes followed by culture of resulting presumptive zygotes up until the blastocyst stage. Our protocol features a unique procedure for washing and moving oocytes and zygotes between their respective dishes using a cell strainer.